This gating method identifies two quadrants (first, and third quadrants) by fitting the data with tmixture model. It is particually useful when the two markers are not well resolved thus the regular quadGate method based on 1d gating will not find the perfect cut points on both dimensions.

gate_quad_tmix(fr, channels, K, usePrior = "no", prior = list(NA),
quantile1 = 0.8, quantile3 = 0.8, trans = 0, plot = FALSE, ...)

## Arguments

fr flowFrame character vector specifies two channels numeric specifies the quantile level(see 'level' in flowClust) for the first quadrant (x-y+) numeric specifies the quantile level see 'level' in flowClust for third quadrant (x+y-) logical whether to plot flowClust clustering results other arguments passed to flowClust

## Value

a filters object that contains four polygonGates following the order of (-+,++,+-,--)