gate_quad_tmix.Rd
This gating method identifies two quadrants (first, and third quadrants) by fitting the data with tmixture model. It is particually useful when the two markers are not well resolved thus the regular quadGate method based on 1d gating will not find the perfect cut points on both dimensions.
gate_quad_tmix( fr, channels, K, usePrior = "no", prior = list(NA), quantile1 = 0.8, quantile3 = 0.8, trans = 0, plot = FALSE, ... )
fr |
|
---|---|
channels |
|
K | |
usePrior | |
prior | |
quantile1 |
|
quantile3 |
|
trans | |
plot | logical whether to plot flowClust clustering results |
... | other arguments passed to flowClust |
a filters
object that contains four polygonGate
s following the order of (-+,++,+-,--)